Table 2 Characterized SSR primers with their diversity characteristics. PN series primers are newly developed primers in the present study
From: Analysis of genetic diversity and population structure in Asparagus species using SSR markers
Primer ID | Primer sequence (5´–3´) | Repeat motif | Ta (°C) | No. of bands | Size range (bp) | PIC | Ho | He |
|---|---|---|---|---|---|---|---|---|
SSR-13 | F:CGACCAGAGAAGGAAGGAG R:CAACCACGCTCATAAGAAC | (TC)11 | 49 | 4 | 590–680 | 0.630 | 0.479 | 0.682 |
SSR-15 | F:ATGATCCCTGAAGTTGTTG R:GTTCCTCTACCAGCCAAG | (TC)20(TG)10 | 47 | 8 | 90–200 | 0.628 | 0.646 | 0.668 |
SSR-22 | F:TAAGCAACTCACTCACTATG R:TGATGTGTGAAGGAGGAGG | (CACT)5 | 48 | 7 | 350–470 | 0.790 | 0.708 | 0.820 |
SSR-37 | F:TATGTTCCTTGCTTCCATG R:CGGTAGAAGTGATTGTGTAT | (AG)11 | 47 | 7 | 140–270 | 0.771 | 0.750 | 0.807 |
SSR-40 | F:GCATATTTCTACTACGCCTCC R:CAAACTAACCCTCAATCACTCG | (ATAGA)5 | 52 | 5 | 400–440 | 0.576 | 0.167 | 0.622 |
SSR-43 | F:CTTGATGGAGCTGGTCTTGT R:TTCTCCACCCTCAATCTCAATAC | (AG)9 | 52 | 3 | 250–400 | 0.630 | 0.458 | 0.696 |
SSR-56 | F:GCTGCTAAGGGATATAGTGCCA R:TATGGTTGCAGAGGATAGGT | (GACAAT)6 | 50 | 5 | 180–350 | 0.760 | 0.542 | 0.800 |
SSR-63 | F:TTAAGTCAGGTGGTGCTCTC R:CTGGATTAGTGGTTGATGATG | (TTGAAAA)54 | 50 | 3 | 140–200 | 0.585 | 0.083 | 0.658 |
SSR-69 | F:GGCTAATTGTGTTGGGAATCG R:CCAACTAATCTACTGACACACG | (AG)14 | 52 | 5 | 220–400 | 0.699 | 0.583 | 0.750 |
SSR-77 | F:GGCCTGCATGTTCTTTATATC R:GCTCATTCTCATCCACTCAT | (CA)12 | 50 | 6 | 230–300 | 0.679 | 0.333 | 0.726 |
SSR-83 | F:GAGTTGAGGCGAGGGACAT R:GTTACTTTCGAGGAGGCCA | (AC)10 | 51 | 4 | 180–400 | 0.644 | 0.208 | 0.702 |
PN3 | F: CCCCTCAAAATCTAACTTCTC R:CAGTTCTACATGCAGATGACC | (GGC)7 | 50 | 4 | 125–160 | 0.389 | 0.208 | 0.411 |
PN4 | F:CCCCCTCTCTAGATATCGTC R:AAGAAGTCGAGGTTTCTGATG | (CT) 12 | 50 | 6 | 500–620 | 0.743 | 0.667 | 0.785 |
PN9 | F:TGAATCTATGGATACCGAAAA R:ATAAAGCCAGACACATCAACA | (TC) 12 | 47 | 5 | 320–450 | 0.767 | 0.646 | 0.804 |
PN10 | F:GATTTTTCTGCCTTCTCTACC R:ATTTTGCCCTTCCATTATCT | (CT)8 | 46 | 5 | 180–230 | 0.616 | 0.625 | 0.664 |
PN12 | F:TGGCTTTTGTGTTGAAATACT R:ACAAATTTTCCCCAATTTTC | (GGT)6 | 44 | 5 | 280–450 | 0.641 | 0.729 | 0.702 |
PN13 | F:TTTCTATTGCCGGAGACTAA R:GCAACTATTCTTCATCCACAG | (GAT)6 | 48 | 6 | 140–170 | 0.782 | 0.938 | 0.817 |
PN14 | F:GACATCGATCTCCTTCTTCTT R:AATCCTGGCATCTGAGGT | (TC)9 | 48 | 5 | 400–600 | 0.763 | 0.333 | 0.802 |
PN15 | F:CCGCTCAGAACTTGTTATTAT R:AATTACAAATAGCCTCTTTTGG | (TA)16 | 47 | 5 | 400–600 | 0.710 | 0.333 | 0.756 |
AGA1 | F: CCGGTGCTTTGATTACTGCT R: GATCATCATCTTGCGCATTG | (AGA)11 | 50 | 3 | 480–600 | 0.536 | 0.479 | 0.598 |
AG5 | F: GATTAATAAAGCGCCGCTGA R:ACATAAGCCCATACTTGCGG | (TC)18 | 50 | 5 | 490–640 | 0.784 | 0.396 | 0.820 |
TC1 | F: AGGTGGAGAACAAATGGCTG R:CGAGCTCAATTGAAATCCATAA | (TC)12 | 49 | 5 | 150–250 | 0.789 | 0.479 | 0.824 |
TC3 | F: CACCATTTCAAATCCCCACT R:GAGGCTAGAGCTCCGCTCAT | (AG)13 | 50 | 7 | 150–350 | 0.775 | 0.750 | 0.809 |
TC8 | F: GGCTAGCCGAAAGAATCTCC R:TCTTCCTCCTCCTCCTCCTC | (CT)10 | 54 | 4 | 130–160 | 0.726 | 0.354 | 0.773 |
Mean | Â | Â | Â | 5 | Â | 0.683 | 0.495 | 0.729 |