Table 1 Description of forward (5′-3′) and reverse (3′-5′) primer sets were designed as two forward specific primers with different 3′ ends (original and underlined substitute nucleotide) and one reverse primer site except for position 1481, two specific reverse and one forward
Position | F 5′ → 3′ | R 3′ → 5′ |
|---|---|---|
467 | TAACTATCTTTGTAGCTCT | ATGAAATATTTACTCATGGG |
TAACTATCTTTGTAGCTCC | ||
586 | AAGCTCTTCTATTCTGGTTT | TCTCTCCCCCGGATAAACCAT |
AAGCTCTTCTATTCTGGTTC | ||
611 | TTCTCTTGGCTATATGGTTT | CTGGAGTGGGAGATCCTTCG |
TTCTCTTGGCTATATGGTTC | ||
737 | ATTGGGTTCAAGCTTTCCCT | TGGAGTGGGAGATCCTTCGT |
ATTGGGTTCAAGCTTTCCCC | ||
747 | AGCTTTCCCCAGCCCCTTCT | GGCATCTTCTTCTGGAAATC |
AGCTTTCCCCAGCCCCTTCC | ||
830 | ACTTCGAAAGTAGCTGCTTT | AACGTATGCTTGCATATTCG |
ACTTCGAAAGTAGCTGCTTC | ||
1481 | CGAAACCAAGAAATAACCCCT | CGGGTTCATTGATATTCCTA |
CGGGTTCATTGATATTCCTG | ||
Actin | TGGTCGTCCAAGACACACTG | CTCTTCAGGGGCAACACGAA |