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Table 3 Comprehensive summary of As_GH and Bo_GH annotations

From: Cloning, nucleotide sequencing, and bioinformatics analyses of growth hormone mRNA of Assaf sheep and Boer goats reared in Egypt

Parameters

As_GH cDNA sequence

Bo_GH cDNA sequence

Length of mRNA

665 bp

774 bp

Predicted promoter

Start 404, end 454, score 0.74

Start 416, end 466, score 0.74

Physical characteristics of growth hormone protein

Length of protein

218 aa

217 aa

Molecular weight

24786.7 bp

24630.5 bp

Chain peptide

28–218 (190 aa)

28–217 (189 aa)

Open reading frame (ORF)

1–218

1–217

Signal peptide

1–27

1–27

Conserved domain

36–216

36–215

Protein properties

Residues

No.

Mole %

Residues

No.

Mole %

Tiny

A,C,G,S,T

63

28.9

A,C,G,S,T

63

29.0

Small

A,B,C,D,G,N,P,S,T,V

96

44.0

A,B,C,D,G,N,P,S,T,V

96

44.2

Aliphatic

A,I,L,V

67

30.7

A,I,L,V

67

30.9

Aromatic

F,H,W,Y

25

11.5

F,H,W,Y

25z

11.5

Non-polar

A,C,F,G,I,L,M,P,V,W,Y

119

54.6

A,C,F,G,I,L,M,P,V,W,Y

119

54.8

Polar

D,E,H,K,N,Q,R,S,T,Z

99

45.4

D,E,H,K,N,Q,R,S,T,Z

98

45.2

Charged

B,D,E,H,K,R,Z

52

23.9

B,D,E,H,K,R,Z

51

23.5

Basic

H,K,R

29

13.3

H,K,R

28

12.9

Acidic

B,D,E,Z

23

   

10.6

Motifs

Position

Position

Somatotropin_1

79–112

79–112

Somatotropin_2

–

190–207

Disulfide bonds

79–190

79–190

208–216

207–215

Cysteine position

18

18

79

79

190

190

208

207

 

216

215