Parameters | As_GH cDNA sequence | Bo_GH cDNA sequence | ||||
---|---|---|---|---|---|---|
Length of mRNA | 665 bp | 774 bp | ||||
Predicted promoter | Start 404, end 454, score 0.74 | Start 416, end 466, score 0.74 | ||||
Physical characteristics of growth hormone protein | ||||||
Length of protein | 218 aa | 217 aa | ||||
Molecular weight | 24786.7 bp | 24630.5 bp | ||||
Chain peptide | 28–218 (190 aa) | 28–217 (189 aa) | ||||
Open reading frame (ORF) | 1–218 | 1–217 | ||||
Signal peptide | 1–27 | 1–27 | ||||
Conserved domain | 36–216 | 36–215 | ||||
Protein properties | Residues | No. | Mole % | Residues | No. | Mole % |
Tiny | A,C,G,S,T | 63 | 28.9 | A,C,G,S,T | 63 | 29.0 |
Small | A,B,C,D,G,N,P,S,T,V | 96 | 44.0 | A,B,C,D,G,N,P,S,T,V | 96 | 44.2 |
Aliphatic | A,I,L,V | 67 | 30.7 | A,I,L,V | 67 | 30.9 |
Aromatic | F,H,W,Y | 25 | 11.5 | F,H,W,Y | 25z | 11.5 |
Non-polar | A,C,F,G,I,L,M,P,V,W,Y | 119 | 54.6 | A,C,F,G,I,L,M,P,V,W,Y | 119 | 54.8 |
Polar | D,E,H,K,N,Q,R,S,T,Z | 99 | 45.4 | D,E,H,K,N,Q,R,S,T,Z | 98 | 45.2 |
Charged | B,D,E,H,K,R,Z | 52 | 23.9 | B,D,E,H,K,R,Z | 51 | 23.5 |
Basic | H,K,R | 29 | 13.3 | H,K,R | 28 | 12.9 |
Acidic | B,D,E,Z | 23 | Â | Â | Â | 10.6 |
Motifs | Position | Position | ||||
Somatotropin_1 | 79–112 | 79–112 | ||||
Somatotropin_2 | – | 190–207 | ||||
Disulfide bonds | 79–190 | 79–190 | ||||
208–216 | 207–215 | |||||
Cysteine position | 18 | 18 | ||||
79 | 79 | |||||
190 | 190 | |||||
208 | 207 | |||||
 | 216 | 215 |