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Table 1 Comparison of the characteristics in second-generation sequencing

From: Metagenomic applications in exploration and development of novel enzymes from nature: a review

Characteristics Roche 454 Ion torrent Illumina
Maximum read length (bp) 1200 400 300
One-way results (Gb) 1 2 1000
Amplification for library construction Yes Yes Yes
Cost/Gb ($) 9538.46 460.00 29.30
Error rate (%) 1 ~ 1 ~ 0.1
Running time (H) 20 7.3 144