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Table 1 Comparison of the characteristics in second-generation sequencing

From: Metagenomic applications in exploration and development of novel enzymes from nature: a review

Characteristics

Roche 454

Ion torrent

Illumina

Maximum read length (bp)

1200

400

300

One-way results (Gb)

1

2

1000

Amplification for library construction

Yes

Yes

Yes

Cost/Gb ($)

9538.46

460.00

29.30

Error rate (%)

1

~ 1

~ 0.1

Running time (H)

20

7.3

144