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Journal of Genetic Engineering and Biotechnology

Table 3 Dioscorin gene DNA marker (DBSPOOA2-913) nucleotide sequence and deduced amino acid sequence alignments and homologous details

From: Physicochemical, enzymatic and molecular characterisation of the storage protein of aerial tuber, Dioscorea bulbifera Linn.

DBSPOOA2 gene

Nucleotide sequence (913 bp)

ACCCTCATGGGTGTCGGTGAGGAGAAGGTGACCCGGCAGCGGAGTTTGTTCGCAGGAGATAGAGGAGTCCAGGGAGACCTACGGTGGGCTGTTTATGAATGTCTACGTGGGGGACTTGATTTTACCAAAGATGATGACGCTGATGAAGGGGGGGAAAAGCACGGGAGGGTGAGTCATGGCTGGTGAAAACTTGCTAACCGCACTCTCTTTCCATCCCATTCTGGCCGCAGCTCTTGCTCCGGCAAGATTGGCTATGACGCTCAACAAGCTCGGCGGATTTGCGGCGACCAATAAGAATTTTCTTCCTTTGGGGAACCACTGGTCGTAGATTTTTACGTCGAGGACAATTACCGGGAGCGAAAAGAAGAGACACACAAGAATGAACGGAGGAGATTGAGGATCAAGAAAAGGAGTTGATTGGAGAAGGAGCAAGAAAGAATTCCAAGGTGCGAATAGATAGTTCATGCCGATATAGTCAGACAACTCGGCGCGAACATGGTGGAACTGGCGGAGGCACCGGAGGAGGAAGAGGAGGGAGGAGAGGAGAACTATTTCTCTGCTTTACCTCCAATTATCATCTAATGTGATTCCCTCCTTCCATAAAATCCATCTTATCTTTATTAAAATGGGTGCTCTTCTACCTGCTGCTAATATTCCATAAACCACTTGATAACCTGAATTTATTTATCTTTATCCTACTGTACAAGGCATCTTAGAAAAAGCGTTGTTTCCTTCTTTCTACCACATCCAACTTGGATTGTTATTCCCTTCCTTTGCAAAATTTATATAAGATTTTTTTTTCCTCTCGAATAACCCTGTGACCCCTTTAGCTGGGTGAACTTTTCACCATTCGGACATGCTCTCCTGTACCATATTTTTCTTTGTGCCTGCTTCCTATTTACCCCTCCCACAA

Translated amino acid sequence (304 amino acids)

TLMGVGEEKVTRQRSLFAGDRGVQGDLRWAVYECLRGGLDFTKDDDADEGGEKHGRVSHGWKLANRTLFPSHSGRSSCSGKIGYDAQQARRICGDQEFSSFGEPLVVDFYVEDNYRERKEETHKNERRRLRIKKRSLEKEQERIPRCEIVHADIVRQLGANMVELAEAPEEEEEGGEENYFSALPPIIICDSLLPNPSYLYNGCSSTCCYSINHLITIYLSLSYCTRHLRKSVVSFFLPHPTWIVIPFLCKIYIRFFFPLEPCDPFSWVNFSPFGHALLYHIFLCACFLFTPPT

Nucleotide sequence alignments

1. Dioscorea japonica voucher Hsu 231 ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (rbcL) gene, partial cds; chloroplast; 89% identity; Accession (JQ733767.1)

2. Dioscorea alata voucher GZY109 ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (rbcL) gene, partial cds; chloroplast; 91% identity; Accession (JX139767.1)

3. Dioscorea nitens voucher YSL 2628 ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (rbcL) gene, partial cds; chloroplast; 89% identity; Accession (JQ733810.1)

Translated amino acid sequences alignments

1. Putative Dioscorin [Oryza sativa Japonica Group]; 41% identity; Accession (BAC99799.1)

2. Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit [Dioscorea esculenta]; 94% identity; Accession (AFC89185.1)

3. Carbonic anhydrase, putative [Neosartorya fischeri NRRL 181]; 45% identity; Accession (XP_001267068.1)

4. Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit [Trebouxia simplex]; 94% identity; Accession (AIJ50559.1)

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