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Journal of Genetic Engineering and Biotechnology

Table 2 Dioscorin gene DNA marker (DBSPOOA1-556) nucleotide sequence and deduced amino acid sequence alignments and homologous details

From: Physicochemical, enzymatic and molecular characterisation of the storage protein of aerial tuber, Dioscorea bulbifera Linn.

DBSPOOA1 gene

Nucleotide sequence (556 bp)

CCTCTCTCTCACTCAACTTTTGCCGCATCCCCCCACCACTCCCCTCCCAGCACTCCCTCTCCTCTCCTTGCCCCCCTTCTACCCTCCTGCCCCCCCGTTCCAAAACTTCTTCTATTCCCCATCTGTTTTTACCAAAGATGATGATCCTTAACTTCTTCTTCCTCCACTTCTCCATTGTACCCCCTTAATTTTACGGCCAGTGGTTCACCAAAGGAAGAAAATTCTTATTGGTCGCCGCAAATCCGACGAGCTTGTTGAGCGTCATAGCCAATCTTGCCGGAGCAAGAGCTGCGGCAAGAATGGGATGGAAAGAGAGTGCGGTTTGCATGTTTTCACTAGCCATGACTCATTACCTCGTGTTATTCGTAACCTTGTATCAGCGTCTACAAGGCAGCAACAGCCTTCCGGCGATGCTCCGTCCGGCTTTCTTCCTCTTCTTCGCCGCACCGAGCATGGCCAGTTTCACCTGGGTGTCAATTTCCGGCGAATTCGACATCTCATGCAAAATGCTTTTCTTCCTTTCTCTTTTCCTCTTCACTTCTCCATTGTACCCCCT

Translated amino acids sequence (184 amino acids)

SLSLNFCRIPPPLPSQHSLSSPCPPSTLLPPRSKTSSIPHLFLPKMMILNFFFLHFSIVPPFYGQWFTKGRKFLLVAANPTSLLSVIANLAGARAAARMGWKESAVCMFSLAMTHYLVLFVTLYQRLQGSNSLPAMLRPAFFLFFAAPSMASFTWVSISGEFDISCKMLFFLSLFLFTSPLYP

Nucleotide sequence alignments

1. Dioscorea alata Dioscorin B (dioB-1) mRNA, complete cds; 100% identity; Accession (AF243526.1)

2. Dioscorea oppositifolia microsatellite Dios23 sequence; 96% identity; Accession (JQ955632.1)

3. Dioscorea cirrhosa isolate PS5034MT03 ribulose-1,5-bisphosphate carboxylase/oxygenase (rbcL) gene, partial cds; chloroplast; 95% identity; Accession (HQ637837.1)

4. Dioscorea alata voucher GZY110 ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (rbcL) gene, partial cds; chloroplast; 95% identity; Accession (JX139768.1)

5. Ipomoea batatas voucher GZY116 ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (rbcL) gene, partial cds; chloroplast; 95% identity; Accession (JX139773.1)

6. Arabidopsis thaliana chromosome 3, complete sequence; 88% identity; Accession (CP002686.1)

Translated amino acids sequence alignments

1. Dioscorin B [Dioscorea alata]; 75% identity; Accession (AAF44711.1)

2. PREDICTED: S-type anion channel SLAH1-like [Solanum tuberosum]; 73 % identity; Accession (XP_006342228.1)

3. Hypothetical protein OsJ_01095 [Oryza sativa Japonica Group]; 71% identity; Accession (EAZ11241.1)

4. S-type anion channel SLAH1 [Arabidopsis thaliana]; 68% identity; Accession (NP_176418.2)

5. Storage protein [Dioscorea cayenensis]; 60% identity; Accession (CAA53781.1)

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