Fig. 2

Column chromatography profile of Dioscorea bulbifera.a Ion-exchange chromatography of dialyzed 70% ammonium sulphate precipitate of the extract of the aerial tuber of Dioscorea bulbifera on DEAE Sephadex A-25 column (elution buffer: 0.05 M Tris-HCl buffer pH 8.3. Adsorbed protein was eluted with 0.05 M Tris-HCl buffer pH 8.3, containing 150 mM NaCl). Column size (1.5 × 20) cm; flow rate 15 ml/h; fraction size 5 ml. b Gel filtration of adsorbed peak from ion-exchange on Sephadex G-75 column (elution buffer: 100 mM Tris-HCl pH 7.9 with 100 mM NaCl). Column size (1.5 × 40) cm; flow rate 27 ml/h; fraction size 3.6 ml