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Table 3 Matrix designed, studied variables, and levels for Laceyella sacchari strain (YNDH) BB factorial experimental design

From: Effective multi-functional biotechnological applications of protease/keratinase enzyme produced by new Egyptian isolate (Laceyella sacchari YNDH)

Trials

X1 (NH4Cl)

X2 (yeast extract)

X3 (NaNO3)

X1X2

X1X3

X2X3

X12

X22

X32

Activity*

1

− 1 (0.08)

− 1 (0.04)

0 (0.026)

1

0

0

1

1

0

1139.2

2

1 (0.18)

− 1 (0.04)

0 (0.026)

− 1

0

0

1

1

0

933.2

3

− 1 (0.08)

1 (0.095)

0 (0.026)

− 1

0

0

1

1

0

999.2

4

1 (0.18)

1 (0.095)

0 (0.026)

1

0

0

1

1

0

977.2

5

− 1 (0.08)

0 (0.065)

− 1 (0.016)

0

1

0

1

0

1

1002.2

6

1 (0.18)

0 (0.065)

− 1 (0.016)

0

− 1

0

1

0

1

666.2

7

− 1 (0.08)

0 (0.065)

1 (0.036)

0

− 1

0

1

0

1

555.2

8

1 (0.18)

0 (0.065)

1 (0.036)

0

1

0

1

0

1

1061.2

9

0 (0.13)

− 1 (0.04)

− 1 (0.016)

0

0

1

0

1

1

1033.2

10

0 (0.13)

1 (0.095)

− 1 (0.016)

0

0

− 1

0

1

1

759.2

11

0 (0.13)

− 1 (0.04)

1 (0.036)

0

0

− 1

0

1

1

911.2

12

0 (0.13)

1 (0.095)

1 (0.036)

0

0

1

0

1

1

921.2

13

0 (0.13)

0 (0.065)

0 (0.026)

0

0

0

0

0

0

851.2

14

0 (0.13)

0 (0.065)

0 (0.026)

0

0

0

0

0

0

854.2

  1. Notes: − 1 (low level), 0 (middle level), and + 1 (high level)
  2. Levels of variables (X1, X2, and X3) presented between brackets are expressed in terms of (%w/v)
  3. *Activity (average of three measurements) was measured as protease using casein substrate and expressed as U/ml