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Table 1 PB experimental design for evaluating factors influencing on protease with keratinolytic activity production by Laceyella sacchari NYDH

From: Effective multi-functional biotechnological applications of protease/keratinase enzyme produced by new Egyptian isolate (Laceyella sacchari YNDH)

Trials

NH4CL, X1

Feather, X2

KH2PO4, X3

K2HPO4, X4

Yeast extract, X5

NaCl, X6

MgSO4.7H2O, X7

CaCl2. X8

CuSO4, X9

MnSO4, X10

NaNO3, X11

Temp. (°C), X12

pH, X13

Inoculum size, X14

Activity*

1

1 (0.1)

1 (1)

− 1 (0.01)

− 1 (0.01)

1 (0.05)

1 (0.1)

1 (0.02)

− 1 (0)

− 1 (0)

− 1 (0)

1 (0.02)

− 1 (45)

1 (8)

1 (5%)

1146.2

2

1 (0.1)

− 1 (0.2)

− 1 (0.01)

1 (0.05)

1 (0.05)

1 (0.1)

− 1 (0)

− 1 (0)

− 1 (0)

1 (0.02)

− 1 (0)

1 (55)

1 (8)

− 1 (1%)

106.82

3

− 1 (0.02)

− 1 (0.2)

1 (1)

1 (0.05)

1 (0.05)

− 1 (0)

− 1 (0)

− 1 (0)

1 (0.02)

− 1 (0)

1 (0.02)

1 (55)

− 1 (6)

1 (5%)

51.02

4

− 1 (0.02)

1 (1)

1 (1)

1 (0.05)

− 1 (0)

− 1 (0)

− 1 (0)

1 (0.02)

− 1 (0)−

1 (0.02)

1 (0.02)

− 1 (45)

1 (8)

1 (5%)

50.62

5

1 (0.1)

1 (1)

1 (1)

− 1 (0.01)

− 1 (0)

− 1 (0)

1 (0.02)

− 1 (0)

1 (0.02)

1 (0.02)

− 1 (0)

1 (55)

1 (8)

− 1 (1%)

34.12

6

1 (0.1)

1 (1)

− 1 (0.01)

− 1 (0.01)

− 1 (0)

1 (0.1)

− 1 (0)

1 (0.02)

1 (0.02)

− 1 (0)

1 (0.02)

1 (55)

− 1 (6)

− 1 (1%)

0.00

7

1 (0.1)

− 1 (0.2)

− 1 (0.01)

− 1 (0.01)

1 (0.05)

− 1 (0)

1 (0.02)

1 (0.02)

− 1 (0)

1 (0.02)

1 (0.02)

− 1 (45)

− 1 (6)

1 (5%)

688.1

8

− 1 (0.02)

− 1 (0.2)

− 1 (0.01)

1 (0.05)

− 1 (0)

1 (0.1)

1 (0.02)

− 1 (0)

1 (0.02)

1 (0.02)

− 1 (0)

− 1 (45)

1 (8)

1 (5%)

32.62

9

− 1 (0.02)

− 1 (0.2)

1 (1)

− 1 (0.01)

1 (0.05)

1 (0.1)

− 1 (0)

1 (0.02)

1 (0.02)

− 1 (0)

− 1 (0)

1 (55)

1 (8)

1 (5%)

38.62

10

− 1 (0.02)

1 (1)

− 1 (0.01)

1 (0.05)

1 (0.05)

− 1 (0)

1 (0.02)

1 (0.02)

− 1 (0)

− 1 (0)

1 (0.02)

1 (55)

1 (8)

− 1 (1%)

312.6

11

1 (0.1)

− 1 (0.2)

1 (1)

1 (0.05)

− 1 (0)

1 (0.1)

1 (0.02)

− 1 (0)

− 1 (0)

1 (0.02)

1 (0.02)

1 (55)

− 1 (6)

− 1 (1%)

0.00

12

− 1 (0.02)

1 (1)

1 (1)

− 1 (0.01)

1 (0.05)

1 (0.1)

− 1 (0)

− 1 (0)

1 (0.02)

1 (0.02)

1 (0.02)

− 1 (45)

− 1 (6)

− 1 (1%)

20.02

13

1 (0.1)

1 (1)

− 1 (0.01)

1 (0.05)

1 (0.05)

− 1 (0)

− 1 (0)

1 (0.02)

1 (0.02)

1 (0.02)

− 1 (0)

− 1 (45)

− 1 (6)

1 (5%)

26.92

14

1 (0.1)

− 1 (0.2)

1 (1)

1 (0.05)

− 1 (0)

− 1 (0)

1 (0.02)

1 (0.02)

1 (0.02)

− 1 (0)

− 1 (0)

− 1 (45)

1 (8)

− 1 (1%)

3.32

15

− 1 (0.02)

1 (1)

1 (1)

− 1 (0.01)

− 1 (0)

1 (0.1)

1 (0.02)

1 (0.02)

− 1 (0)

− 1 (0)

− 1 (0)

1 (55)

− 1 (6)

1 (5%)

42.12

16

− 1 (0.02)

− 1 (0.2)

− 1 (0.01)

− 1 (0.01)

− 1 (0)

− 1 (0)

− 1 (0)

− 1 (0)

− 1 (0)

− 1 (0)

− 1 (0)

− 1 (45)

− 1 (6)

− 1 (1%)

0.00

  1. − 1 low level and + 1 high level
  2. Levels of independent variables (X1–X14) presented between brackets are expressed in terms of (%W/v) or value
  3. X14—inoculum size of 48 h where 1% OD = 0.08 and 5% OD = 0.4, in case of low level − 1 and high level + 1, respectively.
  4. *Activity (average of three measurements) was measured as protease using casein substrate expressed as U/ml.