The present and potential future methods for delivering CRISPR/Cas9 components in plants

Journal of Genetic Engineering and Biotechnology

Table 1 PEG mediated CRISPR/Cas9 components delivery into different plants

Plant name	CRISPR/Ca9 vector or ribonucleoprotein complexes	Targeted genes	Reference
Apple	Cas9-sgRNA ribonucleoprotein complexes	DIPM-1, 2, 4	[15]
Brassica oleracea, Brassica rapa	Cas9-sgRNA ribonucleoprotein complexes	FRI, PDS	[16]
Citrullus lanatus	PHSN1, PHSN2	ClPDS	[17]
Glycine max	pCas9-GmU6-sgRNA, pCas9-AtU6-sgRNA	Glyma08g02290, Glyma12g37050, Glyma06g14180	[18]
Grapevine	Cas9-sgRNA ribonucleoprotein complexes	MLO-7	[15]
Oryza sativum	pRGE3, pRGE6	OsMPK5	[19]
Oryza sativum	pUC19-OsCas9	OsSWEET14, OsSWEET11	[20]
Oryza sativum	pJIT163-2NLSCas9	OsPDS, OsBADH2	[21]
Petunia	Cas9-sgRNA ribonuclease protein complexes (RNPs)	PhACO1	[22]
Physcomitrella patens	pAct-Cas9, psgRNA PpAPT-KO4, PpAPT-KO7	PpAPT	[23]
Solanum tuberosum	Cas9-sgRNA Ribonucleoprotein complexes (RNPs)	GBSS (GT4)	[4]
Triticum aestivum	pCR8-U6-gRNA	TaEPSPS	[24]
Zea mays	p ZmU3-gRNA	ZmIPK	[25]
Zea mays	CT-nCas9	ZmALS1, ZmALS2	[26]