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Fig. 2 | Journal of Genetic Engineering and Biotechnology

Fig. 2

From: The present and potential future methods for delivering CRISPR/Cas9 components in plants

Fig. 2

Schematic representation of CRISPR/Cas9 construct transfer and genome editing processes. CRISPR constructs coated onto gold particles (Gene gun mediated), transferred CRISPR construct into protoplast via PEG, and Agrobacterium strain having CRISPR vector. In the next step, CRISPR constructs are delivered into initial explants. A single plant cells and pictorial representation of gRNA and Cas9 at their target site within the genome. Further, the transformed explants are selected onto appropriate medium in plate. The survived plants were transferred into pots for acclimatization. PCR and Sanger sequencing are generally done for mutation screening

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