Table 2 Experimental plan for the optimization of IAA production by the strain I69: experiment 27, 28, 29, and 30 represent central points
N°Exp | Incubation temperature (°C) | pH | [NaCl] (g/l) | [Tryptophane] (g/l) | Incubation time (days) | [IAA] (μg/ml) |
|---|---|---|---|---|---|---|
1 | 30 | 4.0 | 0.10 | 1 | 15 | 6.90 |
2 | 42 | 4.0 | 0.10 | 1 | 1 | 6.34 |
3 | 30 | 9.0 | 0.10 | 1 | 1 | 18.87 |
4 | 42 | 9.0 | 0.10 | 1 | 15 | 4.91 |
5 | 30 | 4.0 | 1.00 | 1 | 1 | 8.25 |
6 | 42 | 4.0 | 1.00 | 1 | 15 | 2.76 |
7 | 30 | 9.0 | 1.00 | 1 | 15 | 11.34 |
8 | 42 | 9.0 | 1.00 | 1 | 1 | 6.58 |
9 | 30 | 4.0 | 0.10 | 9 | 1 | 5.00 |
10 | 42 | 4.0 | 0.10 | 9 | 15 | 5.80 |
11 | 30 | 9.0 | 0.10 | 9 | 15 | 6.96 |
12 | 42 | 9.0 | 0.10 | 9 | 1 | 4.03 |
13 | 30 | 4.0 | 1.00 | 9 | 15 | 4.30 |
14 | 42 | 4.0 | 1.00 | 9 | 1 | 3.76 |
15 | 30 | 9.0 | 1.00 | 9 | 1 | 27.84 |
16 | 42 | 9.0 | 1.00 | 9 | 15 | 32.41 |
17 | 30 | 6.5 | 0.55 | 5 | 8 | 90.72 |
18 | 42 | 6.5 | 0.55 | 5 | 8 | 2.55 |
19 | 36 | 4.0 | 0.55 | 5 | 8 | 2.98 |
20 | 36 | 9.0 | 0.55 | 5 | 8 | 16.58 |
21 | 36 | 6.5 | 0.10 | 5 | 8 | 119.73 |
22 | 36 | 6.5 | 1.00 | 5 | 8 | 123.27 |
23 | 36 | 6.5 | 0.55 | 1 | 8 | 157.79 |
24 | 36 | 6.5 | 0.55 | 9 | 8 | 108.14 |
25 | 36 | 6.5 | 0.55 | 5 | 1 | 121.93 |
26 | 36 | 6.5 | 0.55 | 5 | 15 | 88.92 |
27 | 36 | 6.5 | 0.55 | 5 | 8 | 118.19 |
28 | 36 | 6.5 | 0.55 | 5 | 8 | 118.27 |
29 | 36 | 6.5 | 0.55 | 5 | 8 | 117.66 |
30 | 36 | 6.5 | 0.55 | 5 | 8 | 117.55 |