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Journal of Genetic Engineering and Biotechnology
Fig. 3 | Journal of Genetic Engineering and Biotechnology

Fig. 3

From: Saccharomyces cerevisiae ER membrane protein complex subunit 4 (EMC4) plays a crucial role in eIF2B-mediated translation regulation and survival under stress conditions

Fig. 3

Halo assay to test the effect of H2O2, ethanol, and caffeine on the growth of eIF2B mutants. Hydrogen peroxide, ethanol, and caffeine halo assays were performed with gcd7-201 gcn2∆, GCD7 gcn2∆, and GCD7 GCN2. S. cerevisiae strains harboring either the empty hc vector, pEG (KG), or the hc/EMC4 gene expressing plasmid were spread on SC medium, containing filter disks soaked in (a) H2O2 (4 mM), (b) ethanol (10%), and (c) caffeine (30 mM) in the presence of either raffinose or galactose. The plates were incubated at 30 °C for 2–3 days

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