Fig. 2

Effect of Emc4p expression on eIF2B mutants. gcd1-502 gcn2∆ mutant strain rescued Ts⁺, Slg⁺, and Gcd⁺ phenotype when transformed with hc/EMC4. (a) gcd1-502 gcn2∆ transformants showing Slg⁺ phenotype at 37 °C were streaked along with gcd1-502 gcn2∆ transformed with hc vector, pEG (KG). (b) Serial dilutions of the transformants showing Slg⁺ phenotype were spotted on SC-Ura and SC medium containing 30 mM-3AT. gcd1-502 gcn2∆ transformed with hc vector, pEG(KG), was spotted as a control. Plates were incubated for 2 days at 30 °C