Table 1 The fission yeast strains utilised in this study
From: Characterisation of unessential genes required for survival under conditions of DNA stress
Strain number | Genotype | Source |
|---|---|---|
SPSC 120 | rad4-116 ura4-D18,leu1-32,ade6-M216 h+ | Collection |
SPSC 573 | rad4-GFP::kanMX cdc25-22 ura3-D18 leu1-32 ade6 h- | Collection |
SPSC 1003 | ade6-M210 leu1-32 ura4-D18 mat1_m-cyhS mt0 rpl42::cyhR(sP56Q) rad4-116::NatMX h- | Collection |
SPSC 1005 | ura4-D18, leu1-32, ade6-704 h− | Collection |
SPSC 1006 | ade6-704 ura4-D18 leu1-32 h+ | Collection |
SPSC 1017 | swi3Δ::KanMX h+ ade6-M216 ura4-D18 leu1-32 h+ | [66] |
SPSC 1018 | mrc1Δ::KanMX ade6-M216 ura4-D18 leu1-32 h+ | [66] |
SPSC 1026 | ade6-M216 leu1-32 ura4-D18 hip1Δ::KanMX h+ | Collection |
SPSC 1029 | rad4::NatMX ura4-D18 h- | Collection |
SPSC 1047 | swi3Δ::KanMX ura4D-18 leu1-32 ade6- h- | This study |
SPSC 1048 | mrc1Δ::KanMX ura4D-18 leu1-32 ade6- h- | This study |
SPSC 1049 | hip1Δ::KanMX ura4-D18 leu1-32 h- | This study |
SPSC 1051 | swi1Δ::KanMX h+ ade6-M216 ura4-D18 leu1-32 h+ | [66] |
SPSC 1056 | ade6-704 ura4-D18 leu1-32 rad52-GFP::KanMX6 h- | Collection |
SPSC 1063 | ade6-704 ura4-D18 leu1-32 rad52-GFP::KanMX6 mrc1::KanMX | This study |
SPSC 1064 | ade6-704 ura4-D18 leu1-32 rad52-GFP::KanMX6 swi3::KanMX | This study |
SPSC 1065 | ade6-704 ura4-D18 leu1-32 rad52-GFP::KanMX6 hip1::KanMX | This study |
SPSC 1073 | ade6-(704 or 210) ura4-D18 leu1-32 rad52-GFP::KanMX6 mat1_m-cyhS, smt0; rpl42::cyhR (sP56Q) rad4-116::NatMX h- | This study |
SPSC 1095 | ura4-D18, leu1-32, ade6-704 leu1::hip1::SV5(x3)::ura4+ h− | This study |
SPSC 1096 | ura4-D18, leu1-32, ade6-704 leu1::swi1::SV5(x3)::ura4+h− | This study |
SPSC 1097 | ura4-D18, leu1-32, ade6-704 leu1::swi3::SV5(x3)::ura4+h− | This study |
SPSC 1098 | ura4-D18, leu1-32, leu1::swi1::SV5(x3)::ura4+swi1Δ::KanMX h− | This study |
SPSC 1099 | ura4-D18, leu1-32, leu1::swi1::SV5(x3)::ura4+swi1Δ::KanMX rad4-116 h+ | This study |
SPSC 1100 | ura4-D18, leu1-32, leu1::hip1::SV5(x3)::ura4+ hip1Δ::KanMX h− | This study |
SPSC 1101 | ura4-D18, leu1-32, leu1::swi3::SV5(x3)::ura4+swi3Δ::KanMX h− | This study |
SPSC 1102 | leu1::swi3::SV5(x3)::ura4+ura4-D18, ade6-704 swi3Δ::KanMX rad4-116 h− | This study |
SPSC 1103 | leu1::hip1::SV5(x3)::ura4+ura4-D18, ade6-704 hip1Δ::KanMX rad4-116 h- | This study |
SPSC 1104 | leu1::swi3::SV5(x3)::ura4+ura4-D18, ade6-704 swi3Δ::KanMX rad4-116 h+ | This study |
SPSC 1105 | leu1::hip1::SV5(x3)::ura4+ura4-D18, ade6-704 hip1Δ::KanMX rad4-116 h+ | This study |
SPSC 1106 | rad52-GFP::KanMX6 leu1::swi1::SV5(x3)::ura4+ura4-D18, ade6-704 swi1Δ::KanMX rad4-116::NatMX | This study |
SPSC 1108 | rad52-GFP::KanMX6 leu1::swi3::SV5(x3)::ura4+ura4-D18, ade6-704 swi3Δ::KanMX rad4-116::NatMX | This study |
SPSC 1110 | rad52-GFP::KanMX6 swi1Δ::KanMX ura4-D18 leu1-32 | This study |
SPSC 1112 | rad52-GFP::KanMX6 leu1::hip1::SV5(x3)::ura4+ ura4-D18, ade6-704 hip1Δ::KanMX rad4-116::NatMX | This study |
SPSC 1114 | leu1::mrc1::SV5(x3)::ura4+ura4-D18, ade6-704 h− | This study |
SPSC 1115 | leu1::mrc1::SV5(x3)::ura4+ura4-D18, ade6-704 mrc1Δ::KanMX h− | This study |
SPSC 1116 | leu1::mrc1::SV5(x3)::ura4+ura4-D18, ade6-704 mrc1Δ::KanMX rad4-116 | This study |