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Fig. 5 | Journal of Genetic Engineering and Biotechnology

Fig. 5

From: Characterisation of unessential genes required for survival under conditions of DNA stress

Fig. 5

The cell morphology of the strains carrying the hip1-V5 allele across 24 h after thiamine induction. The figure shows the cells of the ahip1-V5 strain SPSC 1095, bhip1Δ hip1-V5 strain SPSC 1100 and chip1Δ hip1-V5 rad4-116 strain SPSC 1103 at × 60 magnification stained with DAPI to show the nuclei at the 0 h time point and the 24 h timepoint. An additional time point at 12 h is shown for the SPSC 1103 strain. For SPSC 1095 and SPSC 1100, there was no significant difference between the cells across those 2 time points in terms of cell length, cell shape and nuclear shape. For SPSC 1103, the cells showed two distinct phenotypes that develop from timepoint 0 to 12 to 24 h, for cells to increase or decrease in length. The nuclei of most of these cells showed a fragmentation that becomes more pronounced at the 24 h time point and some cells showed a compacted nucleus. The phenotypical effect of thiamine addition on the hip1-V5 strains across 24 h

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