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Table 1 Effects of different Agrobacterium culture densities and infection period on the survival rate and gus A gene expression in horse gram plants

From: A simple and efficient Agrobacterium-mediated in planta transformation protocol for horse gram (Macrotyloma uniflorum Lam. Verdc.)

Agrobacterium optical cell density (OD)Infection time (min)No. of seeds infectedMean no. of survived plants (%)Mean no. of plants expressing gus A gene
Control010093.33 ± 0.72a0 ± 0f
0.31010083.33 ± 0.42b4.4 ± 0.55d
1510073.67 ± 1.14d5.2 ± 0.09cd
3010060.67 ± 0.56f3.7 ± 0.42d
0.61010081.33 ± 0.67bc7.0 ± 0.15ab
1510078.33 ± 0.83c8.1 ± 0.55a
3010070.00 ± 0.77de4.6 ± 0.40cd
0.81010066.33 ± 0.79e6.1 ± 0.79bc
1510050.00 ± 0.77g4.0 ± 0.33d
3010040.00 ± 0.58h2.0 ± 0.27e
  1. Mean values of three separate trials (±) with standard errors. In each column, numbers with different letters indicate they are considerably different from each other according to Duncan’s multiple range test at a probability level of 5%