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Table 1 Effects of different Agrobacterium culture densities and infection period on the survival rate and gus A gene expression in horse gram plants

From: A simple and efficient Agrobacterium-mediated in planta transformation protocol for horse gram (Macrotyloma uniflorum Lam. Verdc.)

Agrobacterium optical cell density (OD)

Infection time (min)

No. of seeds infected

Mean no. of survived plants (%)

Mean no. of plants expressing gus A gene

Control

0

100

93.33 ± 0.72a

0 ± 0f

0.3

10

100

83.33 ± 0.42b

4.4 ± 0.55d

15

100

73.67 ± 1.14d

5.2 ± 0.09cd

30

100

60.67 ± 0.56f

3.7 ± 0.42d

0.6

10

100

81.33 ± 0.67bc

7.0 ± 0.15ab

15

100

78.33 ± 0.83c

8.1 ± 0.55a

30

100

70.00 ± 0.77de

4.6 ± 0.40cd

0.8

10

100

66.33 ± 0.79e

6.1 ± 0.79bc

15

100

50.00 ± 0.77g

4.0 ± 0.33d

30

100

40.00 ± 0.58h

2.0 ± 0.27e

  1. Mean values of three separate trials (±) with standard errors. In each column, numbers with different letters indicate they are considerably different from each other according to Duncan’s multiple range test at a probability level of 5%