Fig. 1
From: Cryopreservation of shoot apices and callus cultures of globe artichoke using vitrification method
Shootlets of globe artichoke grown on MS medium supplemented with 1 mg/l BA (a), callus cultures were initiated from leaf explants and maintained on MS medium amended with 0.1 mg/l NAA, 0.5 mg/l 2,4-D, and 0.5 mg/l kin (b). Cryotubes taken from liquid nitrogen container (c), cryopreserved shoot apices grown on MS medium + 1 mg/l BA (d), cryopreserved callus inoculum transferred onto MS medium + 0.1 mg/l NAA, 0.5 mg/l 2,4-D, and 0.5 mg/l kin (e) and shootlets developed from cryopreserved shoot apices after 12 weeks of culturing on the recovery medium (f)