Table 1 The composition of different media used in this transformation study
Media | Composition of the media |
|---|---|
Callus induction media | MS medium supplemented with different concentrations of 2,4-D (0, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, 4.0, 5.0 mg/l) |
MS resuspension media | MS salts and vitamins were supplemented with 68 g/l sucrose, 36 g/l glucose, 3 g/l KCl, 4 g/l MgCl2, and dH2O. pH was adjusted to 5.2. |
Infection medium | Infection medium was prepared by adding bacterial culture in MS resuspension medium in an appropriate ratio to adjust OD600 to 0.5. |
Co-cultivation media | MS medium was fortified with 30 g/l sucrose, 10 g/l glucose, 3.0 mg/l 2,4-D and dH2O. pH was adjusted to 5.2. After autoclaving the MS medium different concentrations of filter-sterilized acetosyringone (0 μM, 100 μM, 150 μM, and 200 μM) were added. |
Selection media | MS medium was supplemented with 30 g/l sucrose and 3.0 mg/l 2,4-D followed by adjustment to the required volume with dH2O and pH adjustment to 5.8. After autoclave filter sterilized kanamycin 100 mg/l was added. |
Regeneration media | MS medium supplemented with different concentrations of BA and NAA. |
Rooting medium | MS medium containing no hormone. |