Table 4 The AFLP primer combinations, selective nucleotides, number of total bands polymorphic bands, and fragment size range for Thymus species
From: Genetic diversity analysis of some Egyptian Origanum and Thymus species using AFLP markers
Primer comb. | Selective nucleotides | Number of bands | Polymorphism percentage | Fragment size Range scored | |||
|---|---|---|---|---|---|---|---|
EcoRI | MseI | Total | Polymorphic | Monomorphic | |||
E-AGC/M-CAA | AGC | CAA | 35 | 22 | 13 | 63% | 180–1000 bp |
E-AGC/M-CAC | AGC | CAC | 23 | 8 | 15 | 35% | 250–1000 bp |
E-AGG/M-CTC | AGG | CTC | 62 | 18 | 44 | 35% | 120–830 bp |
E-ACC/M-CAT | ACC | CAT | 51 | 21 | 30 | 35% | 120–790 bp |
Total | Â | Â | 171 | 69 | 102 | Â | Â |
Average | Â | Â | 42 | 17 | 25 | 42% | Â |