Expression of a cucumber alanine aminotransferase2 gene improves nitrogen use efficiency in transgenic rice

Journal of Genetic Engineering and Biotechnology

Table 4 Absorption, agronomic, and grain NUE values in CsAlaAT2 transgenic events

Lines	Character	N fertilizer dosage
Lines	Character	N 50%	N 100%
Wild type	Total biomass (gram/hill)	25.76 ± 1.99	29.83 ± 1.19
	Grain yield/plant (gram/hill)	31.48 ± 3.06	30.55 ± 6.18
	N content in plant	0.28 ± 0.1	0.27 ± 0.03
	Absorption NUE	0.284	0.14
	Agronomical NUE	25.76	14.91
	Grain NUE	31.48	15.28
FAM3	Total biomass (gram/hill)	31.6 ± 3.36	44.13 ± 1.36
	Grain yield/plant (gram/hill)	37.58 ± 3.57	33.02 ± 5.23
	N content in plant	0.39 ± 0.07	0.56 ± 0.13
	Absorption NUE	0.386	0.28
	Agronomical NUE	31.60	22.06
	Grain NUE	37.60	16
	Increase in N uptake efficiency (%)*	34.48	107.4
	Increase in grain yield (%)**	19.40	8
FAM5	Total Biomass (gram/hill)	30.91 ± 0.88	37.74 ± 1.33
	Grain yield/plant (gram/hill)	33.02 ± 3.42	30.63 ± 3.34
	N content in plant	0.43 ± 0.06	0.36 ± 0.12
	Absorption NUE	0.376	0.297
	Agronomical NUE	30.91	18.87
	Grain NUE	33	15.3
	Increase in N uptake efficiency (%)*	48.27	33.33
	Increase in grain yield (%)**	4.9	0.3
FAM13	Total Biomass (gram/hill)	31.78 ± 4	39.39 ± 1.45
	Grain yield/plant (gram/hill)	40.28 ± 3.53	34.09 ± 3.00
	N content in plant	0.32 ± 0.02	0.35 ± 0.07
	Absorption NUE	0.35	0.159
	Agronomical NUE	31.78	19.70
	Grain NUE	40.2	17
	Increase in N uptake efficiency (%)*	10.34	29.63
	Increase in grain yield (%)**	27.95	11.58

*Increase in N uptake efficiency is calculated from the difference between the value of the N content of transgenic event and control (wild-type) divided by the value of N content of control line multiplied by 100%
**Increased in the grain yield is calculated from the difference between the results of grain yield/plant of transgenic lines to control (wild-type) divided by grain yield/plant of control line multiplied by 100%