Table 1 Sequences of primers used in this study for 16s bacterial identification and for heat shock gene (DnaK and HtrA) expression analysis
Primer name | Sequence (5′–3′) |
|---|---|
16s RNA forward | 5′-AACTGGAAGGTGGGGAT-3′ |
16s RNA reverse | 5′-AGGAGGTCCAACCGCA-3′ |
16s RNA-qRT-PCR-F | 5′-TCCTACGGGAGGCAGCAGT-3′ |
16s RNA-qRT-PCR-R | 5′-GGACTACCAGGGTATCTAATCCTGTT-3′ |
DnaK-StF (S. typhimurium) | 5′-CGCTTCCAGGACGAAGAAGT-3′ |
DnaK-StR (S. typhimurium) | 5′-CGAGG TCGTAAACCGCGATA-3′ |
DnaK-ScF (S. choleraesuis) | 5′-CGCTTCCAGGACGAAGAAGT-3′ |
DnaK-ScR (S. choleraesuis) | 5′-CGAGG TCGTAAACCGCGATA-3′ |
HtrA-StF (S. typhimurium) | 5′-CGACGAACAACTCTGGCTCA-3′ |
HtrA-StR (S. typhimurium) | 5′-TTCAAG GGTGTCGAGATGGC-3′ |
HtrA-ScF (S. choleraesuis) | 5′-GAGTGCACTGGCTCTGAGTT-3′ |
HtrA-ScR (S. choleraesuis) | 5′-TTCACC GTGGTGCTACCTTC-3′ |