Table 1 Summary of the purification of protease from S. hydrogenans var. MGS13
Purification stages | Total protein (mg) | Total enzyme activity units | Specific activity (U/mg) | Purification fold | Recovery (%) |
|---|---|---|---|---|---|
Cell-free supernatant | 12,460 | 51,670 | 4 | 1 | 100 |
50% ammonium sulfate precipitation | 840 | 27,230 | 30 | 7 | 52 |
Dialyzed sample | 770 | 22,200 | 40 | 9.7 | 43 |
Sephadex G-100 | 370.25 | 17,500 | 50 | 12 | 34 |