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Journal of Genetic Engineering and Biotechnology
Fig. 2 | Journal of Genetic Engineering and Biotechnology

Fig. 2

From: Endoglucanase gene of M42 aminopeptidase/endoglucanase family from thermophilic Bacillus sp. PW1 and PW2 isolated from Tattapani hot spring, Himachal Pradesh, India

Fig. 2

Isolation of genes encoding cellulase from the thermophiles Bacillus sp. PW1 and Bacillus sp. PW2. a Analysis of genomic DNA of PW1 (lane 1) and PW2 (lane 2) by agarose gel electrophoresis. b PCR amplification of cellulase gene from genomic DNA of PW1 and PW2. Lanes 1 and 2: PCR products showing amplification of cellulase gene of Bacillus sp. PW1 and Bacillus sp. PW2 (~ 1.1 kb), respectively. The size marker (M) bands are indicated (kb). c Dendrogram showing phylogenetic relationship of PW1 and PW2 endoglucanase protein sequences with their representatives showing > 99% homology. The Jaccard’s coefficient scale for genetic relatedness is indicated below phylogenetic tree

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